Uczelnia - Bibliografia Publikacji PIWet-PIB

Ecology driven patterns of herpesvirus spread in cervids in Poland. [AUT.] WOJCIECH SOCHA, ARTUR JABŁOŃSKI, MICHAŁ K. KRZYSIAK, PIOTR KUBIŚ, GEORGE RUSSELL, LARSKA MAGDALENA. Scientific Reports. DOI: 10.1038/s41598-026-61633-0
Cervids are considered as a potential hosts for various human and animal pathogens but studies on herpesvirus infections among these species remain limited. We have used a panherpesvirus PCR to investigate herpesvirus diversity and prevalence and a BoHV-4-specific ELISA to assess exposure to BoHV-4. Among 1257 cervids tested for the herpesvirus DNA, 389 (30.9%) were positive. The prevalence was highest among fallow deer (63.9%), followed by red deer (29.8%) and roe deer (19.4%); among captive animals (42.1%) and in forest districts with low (31.2%) or medium (32.9%) forest coverage. No positive cases were found in moose. BoHV-4 specific antibodies were found in 107 out of 1179 (9.1%) tested cervids. Seroprevalence was highest among roe deer (18.7%) and in regions with the highest forest coverage (13.9%). Two gammaherpesvirus species: Fallow deer …
MGF110-2L deletion prevents IFN-I and inflammatory response, resulting in partial attenuation and protection against virulent ASFV. [AUT.] JULIA GATA-DE-BENITO, MAREK WALCZAK, LIHONG LIU, GONZALO VIGARA-ASTILLERO, KRZESIMIR SZYMANKIEWICZ, MACIEJ KOCHANOWSKI, JACEK ŻMUDZKI, YOLANDA REVILLA, DANIEL PÉREZ-NÚÑEZ. Journal of Virology. DOI: 10.1128/jvi.00636-26
African swine fever virus (ASFV) encodes numerous multigene family (MGF) proteins whose individual contributions to virulence and immune modulation remain poorly understood. Here, we characterize the role of MGF110-2L in shaping innate immunity and disease outcome in vivo. Deletion of MGF110-2L from the virulent Arm/07/CBM/c2 isolate markedly attenuated clinical disease and conferred complete protection against homologous challenge. In macrophages, the MGF110-2L-deficient recombinant induced enhanced type I interferon (IFN) responses, consistent with a role for MGF110-2L in antagonizing antiviral signaling. However, transcriptomic profiling of peripheral blood mononuclear cells revealed that the absence of MGF110-2L prevents the excessive IFN and inflammatory hyperactivation triggered by the parental virus in vivo, establishing a controlled antiviral program associated with reduced pathology. These findings identify MGF110-2L as a functional virulence factor that drives dysregulated innate immune …
Evolutionary dynamics of the multidrug-resistant Salmonella Infantis harbouring the pESI megaplasmid across Europe. [AUT.] PATRICIA ALBA, ELENA LAVINIA DIACONU, VIRGINIA CARFORA, ANTONIO BATTISTI, ALESSIA FRANCO, PIETER-JAN CEYSSENS, CRISTINA GARCIA-GRAELLS, JANNICE SCHAU SLETTEMEÅS, MAGDALENA ZAJĄC, MAGDALENA SKARŻYŃSKA, DARIUSZ WASYL, CLEMENTE LURDES, MANUELA CANIÇA, VERA MANAGEIRO, STEFAN BÖRJESSON, EELCO FRANZ, EMMA ÖSTLUND, ROBERT SÖDERLUND, AURORA GARCIA-FERNANDEZ, LAURA VILLA. Microbial genomics. DOI: 10.1099/mgen.0.001787
Multidrug-resistant Salmonella enterica subsp. enterica serovar Infantis clone, harbouring the pESI megaplasmid, first described in Israel in 2014, is consistently reported in poultry and humans worldwide. This study aimed to investigate the genomic epidemiology of S. Infantis collected by nine European Public Health Institutions from samples of different origins (human, food and animal sources) and understand the evolutionary dynamics of pESI-like in Europe. The resolved pESI-like sequences have also been compared with complete publicly available pESI-like sequences from other countries, in a One Health context. The circulation of a S. Infantis clone in Europe carrying the mosaic megaplasmid pESI-like has been associated with resistance to sulphonamides (sul), tetracycline (tet), streptomycin and spectinomycin (aadA1). In recent years, blaCTX-M-1-positive pESI-like plasmids have been increasingly detected in the extended-spectrum beta-lactamase-producing S. Infantis clone. …
Occurrence of PCDD/Fs and PCBs in Edible Land Snails (Helix pomatia) from Poland: Tissue Distribution and Implications for Food Safety. [AUT.] MONIKA ZIOMEK, JULIA KOWALCZYK, MAREK PAJUREK, SZCZEPAN MIKOŁAJCZYK, WOJCIECH PIETROŃ, KRZYSZTOF SZKUCIK, MICHAŁ GONDEK. Toxics 599. DOI: 10.3390/toxics14070599
Terrestrial snails are recognised as bioindicators of environmental contamination and may represent a source of dietary exposure to persistent organic pollutants (POPs). However, data on polychlorinated dibenzo-p-dioxins, dibenzofurans (PCDD/Fs), and polychlorinated biphenyls (PCBs) in edible land snails remain limited. This study investigated PCDD/Fs, dioxin-like PCBs (dl-PCBs), and non-dioxin-like PCBs (ndl-PCBs) in the foot muscle and hepatopancreas of free-living Helix pomatia (H. pomatia) collected from two regions of Poland. A total of 48 snails (24 per region) were analysed as three pooled replicates per tissue type in each region. Thirty-five congeners were determined using high-resolution gas chromatography coupled with high-resolution mass spectrometry (HRGC-HRMS). Overall, only trace concentrations of selected PCDFs and dl-PCBs were detected, exclusively in hepatopancreas samples. Among PCDFs, only 2,3,7,8-TCDF and 1,2,3,7,8-PeCDF were quantified, whereas PCB 77 was the …
Development and validation of a new SYBR green-based qPCR assay for the broad detection of Novirhabdovirus salmonid (IHNV) genotypes. [AUT.] SUNGJAE KO, EUNSU SONG, MAGDALENA STACHNIK, BERTRAND COLLET, BARTOLOMEO GORGOGLIONE, PO-TSANG LEE, ALEJANDRO J. YÁÑEZ, JOON BUM JEONG, KWANGIL KIM, YOUNGCHUL KIM, DIMITRI RIGAUDEAU, MUN GYEONG KWON, JAE-OK KIM, MIN JAE KIM, JI-MIN JEONG, SUHEE HONG. Aquaculture 744469. DOI: 10.1016/j.aquaculture.2026.744469
Infectious Haematopoietic Necrosis Virus (IHNV) is a WOAH-notifiable pathogen causing severe economic losses in global salmonid aquaculture. While the WOAH-recommended TaqMan assay is the current standard, its diagnostic efficacy is frequently compromised by high execution costs and reduced sensitivity toward specific genotypes, particularly the JS lineage, due to single nucleotide polymorphisms (SNPs) in the probe-binding region. To address these limitations, this study developed a novel SYBR Green-based qPCR assay targeting the highly conserved nucleoprotein (N) gene. Meticulous thermodynamics of a new primer set was optimized by balancing GC content, melting temperature (Tm ), and Gibbs free energy (ΔG)—to ensure broad genotype inclusivity (U, M, E, JN, and JS) while eliminating non-specific amplifications and primer-dimers. The assay demonstrated superior analytical performance, particularly for the JS genotype, yielding a significantly lower Cq …

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