Dietary exposures to food pollutants such as mycotoxins have assumed greater significance due to their adverse effects on production and reproduction of animals andhumans. Citrinin is a is a secondary metabolite produced by several species of the genera Aspergillus, Penicillium and Monascus. This mycotoxin contaminates long­stored food of plant origin and may cause adverse health problems in humans and animals by targeting different organs and systems. The aim of the study was to assessthe androgenic and antiandrogenic effects of citrinin with the use of in vitro yeast­based reporter bioassay that has been developed to study the ability of compounds toinduce human androgen receptor (hAR)– mediated transactivation. Citrinin was studied at 9 concentrations ranging from 0,1 nM to 200 μM alone or in binary mixtureswith 100 nM and 5 μM 17β­testosterone (17βT). Each bioassay was performed two times, for each concentration 6 replicates were applied. The cytotoxicity, EC50,relative androgenic potency (RAP) and antiandrogenic activity of the compounds were assessed. Results showed that citrinin was not cytotoxic in the selectedconcentration range. The compound showed partial androgenic activity in the yeast androgen bioassay (EC50=36.62 mM, RAP=0,229%). Moreover, citrinin showeddose­dependent antiandrogenic response in the presence of 50 and 100 nM of 17βT with the strongest inhibitory effect at the concentration of 50mM. Our studyreveals that citrinin is an endocrine active compound that may disturb androgen receptor­mediated signaling. Further research with the use of in vivo models iswarranted to fill current gaps in knowledge regarding hormonal activity of citrinin and the related health effects in humand and animals. The study was supported by theLeading National Research Centre (KNOW), Scientific Consortium “Healthy Animal­ Safe Food”, Decision of Ministry of Science and Higher Education No 05­1/KNOW/2015