In this paper we aimed at the presentation of antimicrobial resistance among Campylobacter spp. strains isolated from humans, animals and food in EU in 2016. These data were jointly published in the EFSA and ECDC report. The quantitative data was analyzed by using epidemiological cutoff (ECOFF) values. In Campylobacter strains, particularly in C.coli from humans, high to extremely high percentages of isolates were resistant to ciprofloxacin and tetracycline. High resistance to these antimicrobials was also observed in C.coli isolates from poultry and turkeys, whereas much lower levels were recorded for erythromycin. Common resistance to critically important antimicrobials in both human and animal Campylobacter spp. isolates was generally rare.