Anisakis simplex is a zoonotic nematode which can cause human anisakiasis. Furthermore, A. simplex allergens, even of dead larvae, can cause allergic reactions, including anaphylaxis. Due to the frequent occurrence in fish muscles and pathogenicity, A. simplex is a serious danger for consumers of fish products. Therefore, it is necessary to examine fish and fish products for the presence of these parasites before placing them on the market. The purpose of this paper is the review of the methods for A. simplex detection in fish and fishery products. These methods differ according to their effectiveness and type of the target analyte. They also have different suitability for examination of matrices with different properties. Moreover, this paper presents legislation associated with A. simplex detection.