Pasteurella multocida shows significant differences in colony morphology, biochemical activity, antigenic structure and pathogenicity. This bacterium may be primary or secondary factor in P. multocida in European bison in Poland and its identification. Thirteen strains from internal organs of dead or eliminated animals were used in the study. Physiological and biochemical properties were examined with API 20E test (bioMérieux) and by traditional methods according to generally accepted principles. The multiplex PCR (OIE Terrestrial Manual 2012), allowing simultaneous identification of species and determination of capsular type A, B, D or F, was also performed. It was found that examined strains produced catalase, were immotile and did not grow on MacConkey agar. According to biochemical profile in API 20E test, all strains belonged to P. multocida species. On the basis of biochemical examinations, according to Mutters et al. and Bisgaard et al., all examined strains were classified to P. multocida subsp. multocida. In the multiplex PCR, the fragment of 460 bp specific for P. multocida species and the fragment of 1044 bp indicating the presence of capsule type A, were also found in all tested strains.