Introduction: Exposure to chemical contaminants is an important concern worldwide. Most of the toxicological data apply to the effects of chemical contaminants when they are present alone; however, humans are usually exposed to multiple toxic compounds, which might impact health via food or the environment. Enrofloxacin (ENRO) is the most persistent fluoroquinolone in the environment, due to input from veterinary use. This aspect may have important consequences, such as undesirable antibiotic residues and increase in bacterial resistance, which can directly influence consumer's health. Deoxynivalenol (DON) is the most prevalent mycotoxin naturally present in grains and other food commodities. Humans and animals are mainly exposed to DON through cereal‐derived food and feed, respectively. Subsequently, humans can be simultaneously exposed to ENRO and DON through food consumption.