The aim of this study was to present the principles of the organization and evaluation of the results of the first proficiency test for the detection and identification of ruminant DNA in the feed, using real-time PCR, which was organized by the National Reference Laboratory (NRL), in 2016. Proficiency test (PT), is a very important tool to confirm and establish the competence of the laboratory. Basing on the obtained results, we’ve got the evidence that all participating laboratories have achieved very good results (all parameters achieved at 100%).