Uczelnia - Bibliografia Publikacji PIWet-PIB

Characterisation of ORF3, M, N and E gene sequences of porcine epidemic diarrhoea virus from domestic pigs in Poland. [AUT.] OLECH MONIKA, ANTAS MARTA, SZCZOTKA-BOCHNIARZ ANNA. Journal of Veterinary Research (Poland). DOI: 10.2478/jvetres-2022-0051
Porcine epidemic diarrhoea virus (PEDV) is an enteric pathogen causing porcine epidemic diarrhoea and acute gastroenteritis in pigs of all ages. Previous analysis of the viral genome of PEDV in Poland was only based on the spike protein (S) gene sequences and no analysis of other genes has been performed. The aim of this study was to analyse the envelope (E), membrane (M) and nucleocapsid (N) protein and open reading frame 3 (ORF3) gene sequences. Material and Methods: Viral RNA from 18 Polish pig faecal samples that were quantitative reverse transcription PCR-positive for PEDV was analysed in four genomic regions (E, M, N and ORF3). Results: Phylogenetic analysis based on these regions’ sequences revealed that Polish PEDV isolates were highly related and were clustered into group G2a across the four …
Kryzysy dioksynowe oraz przepisy prawne dotyczące dioksyn. [AUT.] PAJUREK MAREK, WARENIK-BANY MAŁGORZATA, MIKOŁAJCZYK SZCZEPAN. Życie Weterynaryjne.
Dioxins (PCDD/PCDFs), and polychlorinated biphenyls (PCBs), still remain of interest to the European Union due to their toxic effects even at very low doses. However, it was only after the so-called “Belgian crisis”, that the European Commission (EC), aiming at a high level of consumers health protection, introduced a number of legal solutions strived at ensuring food and feed safety. The strategy adopted by the European Union allowed for the harmonization of on environmental pollution and the food chain regulatory rules. This article discusses the applicable EU legal regulations regarding dioxins and PCBs in food and feed and the research methods used in the official control of undesirable contaminants.
Determination of clostebol residues in the urine of slaughter animals using liquid chromatography–tandem mass spectrometry. [AUT.] MATRASZEK-ŻUCHOWSKA IWONA, KŁOPOT ALICJA, GRZELAK JUSTYNA, ZDONEK PAULINA. Journal of Veterinary Research (Poland). DOI: 10.2478/jvetres-2024-0070
Introduction: Synthetic anabolic hormones, which may pose a potential risk to human health, should not be used in fattening food-producing animals. Because of the hormonal effects they cause, growth-promoting compounds are banned by legislation in the EU for use in animal husbandry. Consequently, all EU member states are required to conduct monitoring tests on the content and residues of these compounds in prescribed biological matrices to ensure the safety of food consumers. The aim of this research was to develop a liquid chromatography–tandem mass spectrometry method for the detection of the residue of one such anabolic hormone, clostebol in food animal urine. Material and Methods: Clostebol and its marker metabolite residues were determined by a method involving enzymatic hydrolysis, isolation of compounds from urine on a C18 solid-phase extraction (SPE) …
Distribution of carp edema virus in organs of infected juvenile common carp. [AUT.] MATRAS MAREK, STACHNIK MAGDALENA, BORZYM EWA, MAJ-PALUCH JOANNA, REICHERT MICHAŁ. Journal of Veterinary Research (Poland). DOI: 10.2478/jvetres-2023-0049
Introduction: The disease caused by carp edema virus (CEV) manifests with lethargy as a primary sign; this observation inkoi in Japan gained the disease the name koi sleepy disease (KSD). In the years following the discovery of the virus in Japan,KSD cases have been noted in the UK in koi and common carp. Conducting research in order to expand knowledge of theprocesses of distribution of CEV in infected fish organs will be helpful for eradication and diagnostic purposes. Material andMethods: Carp edema virus–affected fish with clinical signs of KSD were experimentally cohabited with common carp fry (30fish). Three fish were euthanised by bath in a 0.5 g L−1 tricaine solution at one week intervals (7, 14, 21 and 28 days postcohabitation). Tissue samples from the brain, gills, spleen, kidney, intestines …
Cytokine secretion in stem cells of cattle infected with bovine leukaemia virus. [AUT.] SZCZOTKA MARIA, KUŹMAK JACEK. Journal of Veterinary Research (Poland). DOI: 10.2478/jvetres-2024-0012
Introduction: Bovine leukaemia virus (BLV) is a Deltaretrovirus responsible for enzootic bovine leukosis, the most common neoplastic disease of cattle. It deregulates the immune system, favouring secondary infections and changes in the blood and lymphatic tissues. Blood homeostasis depends on functional haematopoietic stem cells (HSCs). Bone marrow is populated by these cells, which express CD34+ and CD35+ surface antigens and produce and release cytokines involved in the maintenance of haematopoiesis. The aim of the study was determination of the profile of cytokine production by CD34+ stem cells of cattle naturally infected with BLV. Material and Methods: The HSCs were generated from the blood and lymphoid organs of cows infected with BLV and healthy control cows with immunomagnetic separation and anti-CD34+ monoclonal antibodies. Isolated CD34+ cells were cultivated for two weeks …

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