Uczelnia - Bibliografia Publikacji PIWet-PIB

Impact of wet- and dry-lab workflows on genome assembly, typing and enterotoxin gene detection in whole-genome sequencing of foodborne Staphylococcus aureus. [AUT.] NOÉMIE VINGADASSALON, JACQUES-ANTOINE HENNEKINNE, YACINE NIA, SVEN MAURISCHAT, MARIA BOROWIAK, BRIAN BYRNE, LIONEL KENNETH DYGICO, LAURENTIU CIUPESCU, HUGO GUEDES, GONÇALO ALMEIDA, JOLANTA ROLA, ARKADIUSZ BOMBA, JURIS KIBILDS, ZUZANA KUBICOVA, ELŻBIETA MACKIW, JOANNA KOWALSKA, ALICIA ROMANÓ, LAURA VAZQUEZ ROJO, ANGELO ROMANO, FABIO ZUCCON, TOM VAN NIEUWENHUYSEN, BERT BOGAERTS, KEVIN VANNESTE, MARINA CAVAIUOLO. International Journal of Food Microbiology 111761. DOI: 10.1016/j.ijfoodmicro.2026.111761
Whole genome sequencing (WGS) has revolutionised surveillance and outbreak investigation of pathogenic bacteria in food, but cross-laboratory comparability remains challenging. An Inter-laboratory Comparison Test (ILCT) on the application of whole genome sequencing (WGS) for typing and characterisation of foodborne Staphylococcus aureus isolates was organised by the European Reference Laboratory for Coagulase-Positive Staphylococci (EURL for CPS) from 2023 to 2025. The test involved 12 National Reference Laboratories (NRLs) and evaluated variability in sequencing output, genome assembly metrics, MLST/cgMLST allele calling, and Staphylococcal enterotoxin gene detection introduced at three critical stages of the WGS workflow - DNA extraction, sequencing, and bioinformatics. While DNA extraction protocols showed minimal effects on sequencing quality, major differences arose from bioinformatics pipelines, particularly in contamination detection, reference database use, and assembly tools. Independent sequencing revealed that genome …
First Report of Haplosporidium edule Infection in the Olive-Green Cockle (Cerastoderma glaucum) from the Northern Adriatic Sea: Expanding Host Range and Geographic Distribution. [AUT.] ALESSIA VETRI, ANDREA BASSO, CATERINA D’ONOFRIO, TOBIA PRETTO, EDOARDO TUROLLA, FEDERICA MARCER, ELEONORA FIOCCHI, GIUSEPPE ARCANGELI, LUANA CORTINOVIS, EWA BILSKA-ZAJĄC, VASCO MENCONI. Pathogens 415. DOI: 10.3390/pathogens15040415
Haplosporidium edule is a haplosporidian parasite originally described in the common edible cockle (Cerastoderma edule) along the European Atlantic coast. In this study, we report the first detection of H. edule in the olive-green cockle (Cerastoderma glaucum) from the northern Adriatic Sea, representing both a novel host record and a new geographic occurrence. During a cross-sectional study conducted in May 2019, 90 C. glaucum specimens were collected from three lagoon sites in northeastern Italy. Histological examination of soft tissues revealed haplosporidian developmental stages, including plasmodia, sporoblasts and mature spores, within connective tissues of the mantle, digestive gland, gills and between gonadal tubules in eight individuals from the Goro Lagoon. Molecular characterization based on a fragment of the small subunit ribosomal DNA showed high similarity with the previously published H. edule …
Euro-Asian hybrids of Echinococcus multilocularis from red foxes in northern and northeastern Poland result from secondary contact between long-isolated populations. [AUT.] PAWEŁ GŁADYSZ, DOROTA BIELIŃSKA-WĄŻ, PIOTR WĄŻ, MAŁGORZATA SAMOREK-PIERÓG, JACEK KARAMON, ANNA LASS. Scientific Reports 9986. DOI: 10.1038/s41598-026-40313-z
We investigated the mitochondrial genetic diversity of Echinococcus multilocularis in red foxes from northern and northeastern Poland, and tested the hypothesis that the presence of Asian haplotypes in Poland stems from their introduction from Northeast Asia to Eastern Europe. We targeted the cytochrome c oxidase subunit 1 gene (cox1) of 263 tapeworms from 59 red foxes hunted in selected districts of Pomorskie, Warmińsko-Mazurskie, and Podlaskie Voivodships. We calculated several measures of genetic variation, and constructed median-joining haplotype networks and a RelTime timetree. In addition, a novel alignment-free computational method was applied: the 4D-Dynamic Representation of DNA/RNA Sequences combined with K-means clustering. PCR and sequencing were successful for 252 tapeworms. We identified six haplotypes, three of which were novel. The mismatch distribution was bimodal. The fixation index (FST) between the European …
Golden jackals as hosts of zoonotic parasites: preliminary findings from southern Poland. [AUT.] KATARZYNA BOJARSKA, KATARZYNA KONDRATEK, ALEKSANDRA LESZCZYŃSKA, KAROLINA SIONEK, JENNIFER HATLAUF, JACEK KARAMON, MAŁGORZATA SAMOREK-PIERÓG, HENRYK OKARMA, STANISŁAW ŚNIEŻKO, ANNA DIDKOWSKA, BLANKA ORŁOWSKA, ANNA M. PYZIEL. Journal of Veterinary Research (Poland) 69-74. DOI: 10.2478/jvetres-2026-0018
The golden jackal, Canis aureus, has been rapidly expanding its range across Europe, raising concerns regarding its impact on the health of wildlife, domestic animals and humans. The goal of this study was to examine the parasitological fauna of golden jackals that had recently colonised southern Poland. Material and Methods The direct flotation method with centrifugation was used to search for parasite eggs, and a complex PCR and qPCR were run to detect the presence of tapeworms in nine faecal samples found by dogs and confirmed genetically, and in two samples taken from hunted individuals. The intestine contents of the hunted individuals were also examined using the sedimentation and counting technique. Camera traps were set to assess if the activity of golden jackals overlapped spatially with that of domestic animals. …
Zoonotic Echinococcus granulosus sensu lato genotypes G6 and G7: new insights from the global mitogenome analysis. [AUT.] ANTI BIEDERMANN, TEIVI LAURIMÄE, LIINA ANIJALG, LAURA KAMENETZKY, SILVIA V. SORIANO, NORA PIERANGELI, LORENA E. LAZZARINI, GÉRALD UMHANG, BOLOR BOLD, CHIMEDTSEREN BAYASGALAN, JACEK KARAMON, MAŁGORZATA SAMOREK-PIERÓG, SAMI ŞIMŞEK, FIGEN CELIK, MAJID F. HARANDI, SAEID NASIBI, NAUNAIN MEHMOOD, OLEG CHIHAI, ADRIANO CASULLI, URMAS SAARMA. International Journal for Parasitology 569-579. DOI: 10.1016/j.ijpara.2025.04.014
Cystic echinococcosis is a severe zoonotic disease caused by different species and genotypes belonging to the Echinococcus granulosus sensu lato (s.l.) complex. Among these, genotypes G6 and G7 are the second most common cause of human cystic echinococcosis. One of the very first steps towards understanding the epidemiology of G6 and G7 is to study their genetic and host diversity, population structure and phylogenetic relationships. For this, we sequenced near-complete mitochondrial genomes (12,850–12,856 bp) of 72 new G6 and G7 samples from eight countries and six host species, including humans. By adding 103 sequences from previous studies, the total dataset for further analyses comprised of 175 sequences from 20 countries and seven host species. This is the most comprehensive global mitogenome study of Echinococcus granulosus s.l. G6 and G7 to …

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